This original Research Paper was recently published in the online JACC Journal and authored by: Walid I. Saliba, Kenji Kawai, Yu Sato, Edward Kopesky, Qi Cheng, Saikat Ghosh, Thomas J. Herbst, Rika Kawakami, Takao Konishi, Renu Virmani, Wael A. Jaber, Douglas N. Gibson, Manish Shah, Andrea Natale, Michael Gibson, David R. Holmes and Aloke Finn.
Abstract
Background
Device-related thrombus (DRT) after left atrial appendage closure (LAAC) procedures is a rare but potentially serious event. Thrombogenicity and delayed endothelialization play a role in the development of DRT. Fluorinated polymers are known to have thromboresistant properties that may favorably modulate the healing response to an LAAC device.
Objectives
The goal of this study was to compare the thrombogenicity and endothelial coverage (EC) after LAAC between the conventional uncoated WATCHMAN FLX (WM) and a novel fluoropolymer-coated WATCHMAN FLX (FP-WM).
Methods
Canines were randomized for implantation with WM or FP-WM devices and given no postimplant antithrombotic/antiplatelet agents. The presence of DRT was monitored by using transesophageal echocardiography and verified histologically. The biochemical mechanisms associated with coating were assessed by using flow loop experiments to quantify albumin adsorption, platelet adhesion, and porcine implants to quantify EC and the expression of markers of endothelial maturation (ie, vascular endothelial-cadherin/p120-catenin).
Results
Canines implanted with FP-WM exhibited significantly less DRT at 45 days than those implanted with WM (0% vs 50%; P < 0.05). In vitro experiments showed significantly greater albumin adsorption (52.8 [41.0-58.3] mm2 vs 20.6 [17.2-26.6] mm2; P = 0.03) and significantly less platelet adhesion (44.7% [27.2%-60.2%] vs 60.9% [39.9%-70.1%]; P < 0.01) on FP-WM. Porcine implants showed significantly greater EC by scanning electron microscopy (87.7% [83.4%-92.3%] vs 68.2% [47.6%-72.8%]; P = 0.03), and higher vascular endothelial-cadherin/p120-catenin expression after 3 months on FP-WM compared with WM.
Conclusions
The FP-WM device showed significantly less thrombus and reduced inflammation in a challenging canine model. Mechanistic studies indicated that the fluoropolymer-coated device binds more albumin, leading to reduced platelet binding, less inflammation, and greater EC.
You can read more about the study here: https://www.jacc.org/doi/10.1016/j.jacep.2023.04.013